i-Tube

Supplies:

Slice of bread or citrus peel
750ml Erlenmeyer flask
Media (see Step 4)
1000ml (1 L) graduated cylinder
Several clean milk bottles

1. Prepare a penicillium culture. Expose a slice of bread or a citrus peel to a 70 degree Fahrenheit environment. A blue-green mold should develop.
2. Sterilize the equipment. Place the flask in the oven at 315 degrees Fahrenheit for one hour, or sterilize in a pressure cooker for at least 15 minutes. Wash the milk bottles.
3. Fill the Erlenmeyer flask. Cut the bread or citrus peel into small pieces and fill the flask. Allow to incubate in the dark at 70 degrees Fahrenheit for 5 days. After this incubation period, the flask can be stored in the refrigerator for no more than 10-14 days.
4. Prepare the media. Dissolve the following ingredients, in the order listed, into 500ml of cold tap water: 44.0 grams Lactose Monohydrate, 25.0 grams cornstarch, 3.0 grams sodium nitrate, 0.25 grams magnesium sulfate, 0.50 grams potassium
phosphate mono, 2.75 grams glucose monohydrate, 0.044 grams zinc sulfate, 0.044 grams manganese sulfate. Then add enough cold tap water to make one liter. Use hydrochloric acid to adjust the pH to between 5.0 and 5.5
5. Fill the bottles with media. Fill the milk bottles with this media. Use only enough so that when the bottle is placed in its side, the media does not reach the plug.
6. Add the penicillin spores. First sterilize the bottles of media in a pressure cooker or in the stove, as you did the Erlenmeyer flask. When they have cooled, add approximately one tablespoon of the spores from the bread or citrus peel.
7. Incubate the bottles. Allow the bottles to rest undisturbed on their sides at 70 degrees Fahrenheit for 7 days. If the culture has worked to produce penicillin, it will be in the liquid portion of the media following this incubation period. Finally, filter the media and refrigerate immediately. If you must use it, although this should be avoided, use as soon as possible.

The penicillin made from this experiment should not be used unless it is a survival situation. It is possible for other, toxic molds to grow along with the penicillin, even if you know what you are doing. It is also possible for mold inhibitors to grow, stopping the growth of the penicillin spores.

Views: 23

"Like Us"

Latest Activity

Meryn Mckenzie is now a member of i-Tube
20 hours ago
Watashi Wa Tamago is now a member of i-Tube
Sep 8
SNAKELOC updated their profile
Sep 4
Pyramid Head promoted Bytes's video
Aug 26
Tpungpol is now a member of i-Tube
Aug 22
Bytes posted a video

THE SILENCING OF ALEX JONES

▶️ Donate Now: http://www.freedomainradio.com/donate ▶️ Sign Up For Our Newsletter: http://www.fdrurl.com/newsletter The President Donald Trump hostile mains...
Aug 15
blaze is now a member of i-Tube
Jul 23
Jaz promoted MBD - Tha Last NetBender's video
Jun 17
Jaz is now a member of i-Tube
Jun 15
MBD - Tha Last NetBender commented on Magicwhoha's video
Thumbnail

Make them wet !

"lol well that was pretty straight to the point, lol."
Jun 7
MBD - Tha Last NetBender promoted Magicwhoha's video
Jun 7
Magicwhoha posted a video

Make them wet !

Magicwhoha brings a message from GOD !
Jun 4
Magicwhoha left a comment for Stefan
"Welcome to one the Oddest sites on the internet !"
Jun 2
Magicwhoha commented on Cosmic Joe's status
"! yeah the newer power cords are bs like every company came out with there own connectors to make…"
Jun 2
Cosmic Joe posted a status
"Been having issues with getting a new power cord for my HP notebook so Facebook is limited using my phone and can’t see any birthdays."
Jun 2
Stefan is now a member of i-Tube
Jun 1
Magicwhoha commented on Magicwhoha's video
Thumbnail

Do what you do song

"I'm liking it a lot there ton more options I'm not using still learning about it ... But…"
Jun 1
MBD - Tha Last NetBender commented on Magicwhoha's video
Thumbnail

Do what you do song

"I see you're still working on it, eh? Getting used to it yet?"
Jun 1
MBD - Tha Last NetBender promoted Magicwhoha's video
Jun 1
MBD - Tha Last NetBender commented on MBD - Tha Last NetBender's blog post The Disclaimer...
"Every single time"
Jun 1
Home inspection

© 2018   Created by Pyramid Head.   Powered by

Badges  |  Report an Issue  |  Terms of Service